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Antibiotic resistance in bacterial pathogens is a global threat to human health and wellbeing. Our research aims to understand the ecological and evolutionary aspects of the spread of antibiotic resistance, specifically via mobile genetic elements called conjugative plasmids. These plasmids ofter carry resistance genes which can be transferred between bacteria and even across microbial communities. Human gut microbiome is a crucial element for our health and a scenery for multilayered microbial interactions. Our aim is to define how these interactions affect the spread and maintenance of antibiotic resistance in the gut microbiome.