Algae flourish in fish farming wastewater and produce valuable materials – AI models intensify the process

The Midas research project of the Ģֱ grows microalgae in the wastewater of recirculating fish farms. The algae growing in the wastewater could be made to produce hydrogen. Algae flourish in the wastewater, binding nutrients from the water and thus reducing the impact of fish farming on water systems. To support biotechnological processes, researchers have developed modelling methods to significantly intensify the cultivation of algae.
Syeda Ahmed (vas.), Marco Calderini ja Salli Pääkkönen tarkastelevat vetyä tuottavan Chlamydomonas reinhardtii -viherlevän kasvua.
Syeda Ahmed (left), Marco Calderini and Salli Pääkkönen examine the growth of the hydrogen-producing green alga Chlamydomonas reinhardtii in a laboratory at the Ģֱ.
Published
22.5.2024

Text: Teemu Rahikka | Photos: Teemu Rahikka

The Midas project, a collaboration between the Ģֱ’s Faculty of Information Technology and Department of Biological and Environmental Science and the VTT Technical Research Centre of Finland, studies algae biotechnology and how it could be intensified. 

In the project, green algae are cultivated in connection with the recirculating aquaculture systems. These systems are modern fish farms that use a variety of filtration techniques to allow reuse of the process water in fish tanks. The purpose is for algae to grow and receive the nutrition they need from the wastewater of fish farms. 

Academy Research Fellow Pauliina Salmi from the Faculty of Information Technology leads the consortium. She says that algal biotechnology is a growing field of technology that also has significant commercial potential. 

“You can get almost everything from algae that you get from fossil oil,” Salmi says.

 “Algae can be used as food and a source of pigments. As they grow, algae bind plenty of carbon dioxide and nutrients dissolved in water. Moreover, you can get hydrogen from them and use it in the production of fuels.” 

The Midas project focuses especially on cultivating the species of green algae that produce hydrogen and testing new algae for the production of hydrogen. Hydrogen raises interest both as a fuel and for use in the refining processes of fuels. 

Algae can give salmonids a red colour

Producing hydrogen with algae is not as efficient as with the most important industrial methods so the scale of hydrogen production in the process is much smaller. However, in the process used in the research, algae grows as a side product of the fish farming industry as if by itself. 

“In certain living conditions, algae produce hydrogen naturally and the needed technology is simple,” Salmi says. 

“On the other hand, the growth of algae must be monitored continuously so that it is possible to improve the efficiency of cultivation and ensure that the algae are growing in the desired manner so that they will have a good concentration of valuable biomolecules.” 

In addition to producing hydrogen, the project has, among others, tested the production of valuable astaxanthin pigment from green algae. Astaxanthin produces the red meat colour of salmon. Currently, the astaxanthin used in the farming of salmonid fish is synthetic, but the pigment produced by green algae would decrease the need to use synthetic products. 

Minna Hiltunen (vas.), Hemanta Timilsina ja Katja Pulkkinen tarkastelevat Haematococcus pluvialis -viherlevän astaksantiinituotantoa.
Haematococcus pluvialis green algae produce astaxanthin in the laboratory. The production process is monitored by Minna Hiltunen (left), Hemanta Timilsina and Katja Pulkkinen.

Automated growth monitoring with artificial intelligence 

In the project, the role of the Faculty of Information Technology is to develop monitoring methods to support the process management so that the quality of the algal biomass can be monitored effectively. 

The growth of the algae and the production of biomolecules is monitored using spectroscopy and artificial intelligence. The purpose is to automate the whole process and for artificial intelligence to identify the issues that possibly threaten the growth of algae and, vice versa, the issues that are beneficial for their growth. 

“In the current commercial production of algae, monitoring is arranged by taking individual samples from the reactors in which algae grow,” Salmi says. “After that, the samples are analysed in a laboratory. Monitoring this way is expensive and haphazard, and samples cannot be taken from very large volumes of algae.” 

The methods developed in the project are non-invasive. No sampling is needed and sensors monitor the quality of the algal culture without disrupting the growth.  

If the new methods prove to be efficient, they will be much more effortless and efficient than those currently in commercial use. 

“Monitoring based on artificial intelligence and, for example, a spectral camera is efficient because it enables the monitoring of the whole algal culture in real time,” says Salmi. “This makes it possible to react to different changes in algal growth exactly at the right time.” 

In principle, these monitoring methods could also be used for traditional algal cultures. However, in this project the focus is on green algae grown in connection of recirculating fish farming. 

Syeda Ahmed (takana vas.), Salli Pääkkönen, Katja Pulkkinen, Marco Calderini, Minna Hiltunen, Juhani Pirhonen, Pauliina Salmi (edessä vas.) ja Hemanta Timilsina kuuluvat tutkimusryhmään.
Syeda Ahmed (top left), Salli Pääkkönen, Katja Pulkkinen, Marco Calderini, Minna Hiltunen, Juhani Pirhonen, Pauliina Salmi (bottom left) and Hemanta Timilsina explore green technologies.

Practical applications of the circular economy support environmental protection 

Contrary to traditional fish farming, in the recirculating fish farms the fish are grown in tanks that use only a small amount of new water while the used water is filtered and recirculated back to the fish with pumps. 

Recirculating aquaculture enables nutrients to accumulate in the system to the extent it is possible to grow algae. 

“The perspective of circular economy in this project comes especially from how we utilise waste from a system to produce new biomass,” says Senior Lecturer Juhani Pirhonen from Department of Biological and Environmental Science. “This means that when fish are fed, their metabolism produces nutrients in the water, and these nutrients are utilised for the algal culture.” 

Instead of chemically cleaning the nutrient-rich water that comes as a by-product of fish farming, the algae growing in the wastewater absorbs the nutrients for better use. 

As such, the mere cultivation of algae as part of recirculating aquaculture would be beneficial, but algae are also good carbon sinks. 

“I would say that in this project we build a concept that we can utilise commercially in fish farming and possibly also elsewhere, where carbon dioxide is a by-product of industry,” Salmi says.

This project has received funding from the European Union – NextGenerationEU instrument.